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KMID : 0613820210310070609
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Journal of Life Science
2021 Volume.31 No. 7 p.609 ~ p.616
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Evaluation of Bioassay Methods to Assess Bacterial Soft Rot Resistance in Radish Cultivars
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Afroz Tania
Hur On-Sook
Ro Na-Young
Lee Jae-Eun
Hwang Ae-Jin
Kim Bich-Saem
Assefa Awraris Derbie
Rhee Ju-Hee
Sung Jung-Sook
Lee Ho-Sun
Hahn Bum-Soo
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Abstract
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Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the destructive diseases of radish (Raphanus sativus) in Asian countries. The objective of this study was to establish an efficient bioassay method for the evaluation of bacterial soft rot resistance in commercial radish cultivars. First, an efficient bioassay method for examining resistance to bacterial soft rot in commercial radish cultivars was investigated. Six commercial radish cultivars were tested under various conditions: two temperatures (25℃ and 30℃), three inoculations methods (drenching, spraying, and root dipping), and two growth stages (two- and four-leaf stages). The results suggested that spraying with 1×106 cfu/ml of bacterial inoculums during the four-leaf stage and incubating at 30℃ could be the most efficient screening method for bacterial soft rot resistance in commercial radish cultivars. Second, we investigated the degree of resistance of 41 commercial radish cultivars to five Pcc isolates, namely KACC 10225, KACC 10343, KACC 10421, KACC 10458, and KACC 13953. KACC 10421 had the strongest susceptibility in terms of moderately resistant disease response to bacterial soft rot. Out of the 41 radish cultivars, 13 were moderately resistant to this pathogen, whereas 28 were susceptible. The moderately resistant radish cultivars in this investigation could serve as resistance donors in the breeding of soft rot resistance or could be used to determine varietal improvement for direct use by breeders, scientists, farmers, researchers, and end customers.
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KEYWORD
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Bacterial soft rot, disease, evaluation, Raphanus sativus, resistance
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